| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Toquica, Iyanna (12)
|
CTZEUS |
35.55 |
34.75 |
-0.80 |
| 2 |
Serrano, Joshua (11)
|
MRYMID |
41.11 |
39.59 |
-1.52 |
| 3 |
Herten, Christina (12)
|
MRYMID |
43.07 |
40.54 |
-2.53 |
| 4 |
Dominick, Kelcie C (12)
|
MRYMID |
39.21 |
40.66 |
1.45 |
| 5 |
Wozniak, Natalie (11)
|
MRLIFE |
45.27 |
40.85 |
-4.42 |
| 6 |
Palencia Escalante, Javier (12)
|
CTZEUS |
49.69 |
41.07 |
-8.62 |
| 7 |
Garcia-Reyes, Johann (11)
|
CTZEUS |
52.10 |
45.33 |
-6.77 |
| 8 |
Henry, Justin R (12)
|
MRNFS |
44.00 |
46.27 |
2.27 |
| 9 |
De Silva, Riaan (11)
|
CTZEUS |
|
47.13 |
- |
| 10 |
Martocci, Ayla T (11)
|
CTZEUS |
57.24 |
51.91 |
-5.33 |
| 11 |
Exantus, Claude J (12)
|
MRNFS |
56.32 |
53.68 |
-2.64 |
| 12 |
Kim, Caden (11)
|
MRLIFE |
|
59.49 |
- |
| 13 |
Veliz, Sharon J (12)
|
MRNFS |
|
1:01.13 |
- |
| 14 |
Sesay, Adrian A (11)
|
MRNFS |
|
1:03.14 |
- |
|
Campbell, Andrea (12)
|
MRNFS |
|
DQ |
- |